Separation of Glutamate Dehydrogenases of Coprinus cinereus on Polyacrylamide Gels
نویسندگان
چکیده
منابع مشابه
Factors affecting the amount and the activity of the glutamate dehydrogenases of Coprinus cinereus.
Kinetic analyses done with cell-free extracts of this basidiomycete fungus showed that the NADP-linked glutamate dehydrogenase exhibited positively co-operative interactions with the substrates 2-oxoglutarate and NADPH, negatively co-operative kinetics with NADP+ and was extremely sensitive to inhibition of deamination activity by ammonium and/or ammonia. The NAD-linked enzyme showed positive c...
متن کاملSeparation of the complementary strands of DNA fragments on polyacrylamide gels.
32P-labeled (in vivo) phiX174 RFI DNA was restricted by Hinc II. Three aliquots of the same digest: a) nondenatured, b) heat denatured, and c) denatured by 5 mM Me-HgOH were analyzed on 3-15% acrylamide gel gradients or on 3% gels with reduced N,N'-methylene-bis-acrylamide. The autoradiography of the gels showed that the nondenatured sample migrates two times faster than the denatured samples. ...
متن کاملThe origin of multiple B mating specificities in Coprinus cinereus.
Mushrooms, such as Coprinus cinereus, possess large families of pheromones and G-protein-coupled receptors that are sequestered at the B mating-type locus and whose function is to confer vast numbers of different mating types. This ability results from complex patterns of cognate and noncognate pheromone/receptor pairings, which potentially offer a unique insight into the molecular interaction ...
متن کاملDe novo methylation of repeated sequences in Coprinus cinereus.
We have examined the stability of duplicated DNA sequences in the sexual phase of the life cycle of the basidiomycete fungus, Coprinus cinereus. We observed premeiotic de novo methylation in haploid nuclei containing either a triplication, a tandem duplication, or an ectopic duplication. Methylation changes were not observed in unique sequences. Repeated sequences underwent methylation changes ...
متن کاملSome characteristics of extracellular proteinases from Coprinus cinereus
Spent culture medium contained five electrophoretically distinct proteolytic activities when Coprinus c;llereus was grown in culture medium which contained protein as sole source of nitrogen. The enzymes were separated by ultrafiltration, gel filtration and ionexchange chromatography into two groups designated EL and AD fractions. The EL fraction passed through a DE-52 column with eluant, and c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1977
ISSN: 0022-1287
DOI: 10.1099/00221287-98-1-281